Synthesis of Arbitrary Interference Patterns Using a Single Galvanometric Mirror and Its Application to Structured Illumination Microscopy.

ABSTRACT: Structured illumination microscopy (SIM) overcomes the diffraction limit of optical microscopy by projecting finely spaced interference fringes with different orientations and phases onto the sample and imaging the result. A major challenge of SIM is to generate the different illumination patterns with a high contrast and switching speed, which commonly requires expensive devices and the sacrifice of illumination power efficiency. We present a new way of generating interference patterns for 2D and 3D SIM achromatically, with high speed and high power efficiency, using only one moving part. The interference patterns are created by a common-path interferometer, with the orientation, polarization, and phase of interference patterns controlled by a single galvanometric mirror. We characterize

SUBMITTER: Guo K 

PROVIDER: S-EPMC12272674 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature