Development of stromal corneal grafts using a novel decellularization method with sodium cocoyl glutamate on <i>GGTA1</i>/<i>CMAH</i>/<i>β4GalNT2</i> knock-out porcine corneas.

ABSTRACT:

Background

Diseases of the human cornea often necessitate corneal transplantation. However, donor corneas are not always readily available, leaving many patients waiting for donated corneas. Porcine corneas are a promising alternative to human donor corneas due to their close anatomical and physiological similarities. In this study, we produced GGTA1/CMAH/β4GalNT2 knockout pigs [triple knockout (TKO)] to minimize immune rejection. We investigated the efficacy and safety of a novel corneal decellularization process using sodium cocoyl glutamate (SCG) and supernuclease (SN).

Methods

We harvested cornea stromal grafts from 2-month-old TKO pigs, decellularized them using SCG and SN. The optical transparency, DNA content, collagen content, glycosaminoglycan c

SUBMITTER: Kang JT 

PROVIDER: S-EPMC12272801 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature