Single-subunit RNA polymerases, KpnP, Ro45Iw, and CD23823, with precise terminal synthesis.

ABSTRACT: T7 RNA polymerase (RNAP) is a highly active single-peptide polymerase that is widely used for in vitro RNA synthesis. T7 RNAP synthesizes RNA with a heterogeneous 3'-end, which sometimes causes problems. To date, some RNAPs (e.g., KP34 and Syn5) that synthesize RNA with more precise 3'-ends have been reported. However, they have their own characteristics and thus do not fully substitute T7 RNAP. To increase the number of usable RNAP repertoires, we searched for other RNAPs and their promoters in the phage RNAP database. From the first screening of nine RNAPs, we selected three RNAPs, named KpnP, Ro45Iw, and CD23823, together with their promoter sequences. Characterization of recombinant RNAPs revealed that compared to T7, these three polymerases exhibited similar RNA synthesis activities b

SUBMITTER: Takatsuki H 

PROVIDER: S-EPMC12272871 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature