A method for purifying and concentrating Mycobacterium tuberculosis in sputum specimens: The double-membrane filtration and concentration method (DMFCM).

ABSTRACT:

Background

Tuberculosis (TB) is an infectious disease that poses a global hazard to public health. Clinically, the small number and low concentration of Mycobacterium tuberculosis (M.tb) in some specimens make TB difficult to detect in the laboratory and affect the sensitivity of diagnosis.

Methods

M.tb in clinical sputum specimens was purified and concentrated using the double membrane filtration concentration method (DMFCM), which was compared with existing methods and evaluated for application.

Results

DMFCM removes 40-80% of impurities from 80.53% of specimens while concentrating M.tb by up to 3.366 times. In microscopy examination, compared with the direct smear method (DSM), the sensitivity with DMFCM of the test was increased from 58.33% to

SUBMITTER: Liu D 

PROVIDER: S-EPMC12272938 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature