Development of a multi-targeted real-time PCR assay for the detection of the grapevine pathogen Xylophilus ampelinus.

ABSTRACT:

Background

Xylophilus ampelinus is a plant pathogenic bacterium that causes bacterial blight in grapevines, which can lead to severe yield losses and economic damage. Owing to its fastidious growth on culture media, detection is primarily based on molecular methods. However, existing tests have produced inconsistent results, particularly when used to detect latent infections and non-validated matrices. There is a risk of false-positive results, with economic consequences such as restrictions on international trade. To enhance the diagnostics of X. ampelinus, a genome-informed approach was utilised to identify new potential targets for specific detection. On the basis of these sequences, multiple real-time PCR assays were designed, and their specificity and sensitivity were assessed

SUBMITTER: Bencic A 

PROVIDER: S-EPMC12273336 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature