Photodynamically tunable ROS-generating hydrogels for accelerated tissue regeneration.

ABSTRACT: Wound healing progresses through many key cellular activities, including fibroblast and keratinocyte proliferation and angiogenesis. This study explored the wound-healing potential of reactive oxygen species (ROS)-generating hyaluronic acid (HA) hydrogels. We fabricated a chlorin e6-conjugated HA (Ce6-HA) hydrogel that generates ROS when subjected to irradiation from an LED light source. In vitro studies revealed that the ROS generated by the Ce6-HA hydrogels enhanced the proliferation of fibroblasts and keratinocytes. Further, the fibroblasts were found to have high levels of intracellular ROS, elevated expression of p-ERK1/2, p-p38 MAPK, p-Akt, and cyclin D1 proteins, and enhanced collagen deposition. Moreover, the Ce6-HA hydrogel also promoted endothelial angiogenesis in vitro

SUBMITTER: Hong SH 

PROVIDER: S-EPMC12273516 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature