Delayed urea differential enhancement CEST (dudeCEST)-MRI with T₁ correction for monitoring renal urea handling.

ABSTRACT:

Purpose

We demonstrate a method of delayed urea differential enhancement CEST for probing urea recycling action of the kidney using expanded multi-pool Lorentzian fitting and apparent exchange-dependent relaxation compensation.

Methods

T₁ correction of urea CEST contrast by apparent exchange-dependent relaxation was tested in phantoms. Nine mice were scanned at 7 Tesla following intraperitoneal injection of 2M 150 μL urea, and later saline. T₁ maps and Z-spectra were acquired before and 20 and 40 min postinjection. Z-spectra were fit to a 7-pool Lorentzian model for CEST quantification and compared to urea assay of kidney homogenate. Renal injury was induced by aristolochic acid in 7 mice, and the same scan protocol was performed.

Results

Apparen

SUBMITTER: Shin SH 

PROVIDER: S-EPMC12273596 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature