BLIMP1 negatively regulates IL-2 signaling in T cells.

ABSTRACT: Interleukin-2 (IL-2) regulates immune homeostasis by fine-tuning the balance between effector and regulatory T (T_reg) cells. To identify regulators of IL-2 signaling, we performed genome-wide CRISPR-knockout screening in IL-2-dependent cells derived from a patient with adult T cell leukemia (ATL) and found enrichment of single guide RNAs targeting PRDM1, which encodes B lymphocyte-induced maturation protein 1 (BLIMP1). BLIMP1 inhibits IL-2 production by T cells; however, its role in IL-2 signaling remains unknown. Here, we show that overexpressing Prdm1 down-regulated IL-2 signaling, whereas Prdm1-deficiency enhanced IL-2 signaling in mouse CD4⁺ T cells and T_reg cells with augmented IL-2 signaling in T cells from influenza-infected mice

SUBMITTER: Roy S 

PROVIDER: S-EPMC12273773 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature