6-Shogaol Alleviates Endotoxin-Induced Uveitis via Activation of HIF-1α to Regulate Inflammatory Responses and Endoplasmic Reticulum Stress.

ABSTRACT:

Purpose

6-Shogaol (6-S) has demonstrated anti-inflammatory effects in various disease models; however, its effects in uveitis have not been investigated. This research intends to investigate the therapeutic mechanisms of 6-S in acute uveitis.

Methods

An endotoxin-induced uveitis (EIU) mouse model was developed to assess the therapeutic potential of 6-S for uveitis. Disease severity was evaluated through anterior segment evaluation, clinical scoring, and pathology staining. RNA sequencing (RNA-seq) and network pharmacology explored the potential molecular mechanisms. In vitro, BV2 cells were stimulated by lipopolysaccharide. The mRNA and protein levels of pro-inflammatory cytokines, endoplasmic reticulum (ER) stress markers, and hypoxia-inducible factor 1-alpha (HIF-1α) in ey

SUBMITTER: Yi W 

PROVIDER: S-EPMC12273885 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature