Redox buffering and H₂O₂ orchestrate the vegetative development of Marchantia polymorpha.

ABSTRACT: Redox processes and reactive oxygen species (ROS) signaling play not only a crucial role in stress responses but also in angiosperm development. However, the specific mechanisms by which redox homeostasis regulates meristems and growth in non-vascular plants remain poorly understood. Here, we demonstrate the applicability of the roGFP2-hGrx1 and HyPer7 redox-biosensors for imaging dynamic glutathione (GSH) and H₂O₂ redox states in the liverwort Marchantia polymorpha. RoGFP2-hGrx1 microscopy, together with analysis of knockdown plants of the GAMMA GLUTAMYLCYSTEINE SYNTHETASE gene MpGSH1, unveiled a more reduced GSH redox potential (E_GSH) in the meristematic region and a more oxidized state in differentiated thallus tissues. Rather than absolute E_GSH

SUBMITTER: Kock C 

PROVIDER: S-EPMC12274077 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature