Single-cell analysis reveals CD34⁺CD90⁺ endothelial cells promote tumor metastasis in gallbladder cancer.

ABSTRACT: Gallbladder cancer (GBC) is the most common malignancy of the biliary tract, with high metastasis incidence and extremely low survival rate. The tumor endothelial cells (TECs) are fundamental components in the tumor microenvironment and significantly contribute to various tumor progression; however, the roles of TECs in GBC are poorly understood. Here, using single-cell RNA sequencing, we identify a GBC-enriched endothelial population-CD34⁺CD90⁺ ECs (SAEndo2). The CD34⁺CD90⁺ endothelial subset correlates with patients' poor prognosis and liver metastasis. In vitro and in vivo experiments suggest that CD34⁺CD90⁺ ECs promote the GBC cell migration and metastasis, showing EndoMT properties. Moreover, CD34⁺CD90^+<

SUBMITTER: Hou M 

PROVIDER: S-EPMC12274528 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature