Distribution patterns of N6-methyladenine in the rye genome.

ABSTRACT: N6-methyladenine (6 mA) has emerged as a potential epigenetic marker in eukaryotic genomes, yet its precise distribution patterns and biological functions in plant genomes are still not fully understood. In this study, we investigated the occurrence, global levels, and distribution patterns of 6 mA in four rye species: Secale cereale, S. strictum, S. sylvestre, and S. vavilovii. Using multiple complementary approaches, i.e., ELISA, ultra-performance liquid chromatography tandem mass-spectrometry (UPLC-MS/MS), immunofluorescence, and high-throughput sequencing (PacBio and MeDIP-seq), 6 mA was detected in all the examined species. The level of 6 mA in the genomic DNA of the rye species, depending on the species and organ, ranged from approximately 1 to 10 per million nucleotides. Immunofluor

SUBMITTER: Kalinka A 

PROVIDER: S-EPMC12274537 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature