Systematic comparison and base-editing-mediated directed protein evolution and functional screening yield superior auxin-inducible degron technology.

ABSTRACT: Biological mechanisms are inherently dynamic, requiring precise and rapid manipulations for effective characterization. Traditional genetic manipulations operate on long timescales, making them unsuitable for studying dynamic processes or characterizing essential genes, where chronic depletion can cause cell death. We compare five inducible protein degradation systems-dTAG, HaloPROTAC, IKZF3, and two auxin-inducible degrons (AID) using OsTIR1 and AtFB2-evaluating degradation efficiency, basal degradation, target recovery after ligand washout, and ligand impact. This analysis identifies OsTIR1-based AID 2.0 as the most robust system. However, AID 2.0's higher degradation efficiency comes with target-specific basal degradation and slower recovery rates. To address these limitations, we emplo

SUBMITTER: Xing 

PROVIDER: S-EPMC12274581 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature