Influenza A virus disruption of dendritic cell-natural killer cell crosstalk impacts activation of naive helper and cytotoxic T cell subsets.

ABSTRACT: Dendritic cells (DCs) and natural killer (NK) cells engage in reciprocal interactions to trigger an efficient innate immune response while governing downstream adaptive immunity. Here, we used an ex vivo autologous human primary immune cell coculture system of DCs and NK cells to examine their impact on naïve CD4+ and CD8+ T cell (CD3+CD45RA+CD197+) activation in response to influenza A viral (IAV) infection. Using multiparameter flow cytometry, we observed that culturing T cells with both DCs and NK cells enhanced CD69 expression on CD4+ and CD8+ T cells, increased CD25 on CD4+ T cells, and promoted CD8+ T cell proliferation, compared with cultures with only NK cells or DCs. When DCs were exposed to the pandemic A/California/07/2009 (H1N1) strain or the A/Victoria/361/2011 (H3N2) strain,

SUBMITTER: Morson CA 

PROVIDER: S-EPMC12274644 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature