Interleukin-27-producing cells in gram-negative neonatal sepsis display diverse phenotypes and functions in the liver.

ABSTRACT: Neonates have increased vulnerability to life-threatening infections due to the distinct immune landscape. Interleukin (IL)-27 is a key component of this immune profile that we have previously shown to be elevated in both newborn humans and mice. IL-27 continues to increase in the serum and tissues consistent with poor outcomes during gram-negative neonatal bacterial sepsis. Presently, we dissected the IL-27 producer profile at a single-cell level using IL-27p28eGFP reporter mice in our previously established model of neonatal sepsis with luciferase-expressing K1-encapsulated Escherichia coli. Whole animal imaging regionally highlighted the spleen, liver, and lungs as key infection sites by bacterial luminescence. Flow cytometry showed that IL-27 producers increased significantly in the li

SUBMITTER: Vance JK 

PROVIDER: S-EPMC12274645 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature