Characterization of extensive diversity in immunoglobulin light chain variable germline genes across biomedically important mouse strains.

ABSTRACT: The light chain immunoglobulin (IG) genes of inbred mouse strains are poorly documented in current gene databases. We previously showed that IG heavy chain (IGH) loci of wild-derived mouse strains, representing the major mouse subspecies, contained 247 IGH variable (V) sequences not curated in the International ImMunoGeneTics (IMGT) information system database, commonly used for adaptive immune receptor repertoire sequencing (AIRR-seq) analysis. Despite containing levels of polymorphism similar to the IGH locus, the germline gene content and diversity of the light chain loci (kappa, IGK; lambda, IGL) have not been comprehensively cataloged. To explore the extent of germline light chain repertoire diversity across mouse strains commonly used in the biomedical sciences, we performed AIRR-seq

SUBMITTER: Kos JT 

PROVIDER: S-EPMC12274646 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature