Exploring the impact of pre-analytical processes on the determination of neuron-specific enolase in serum samples.

ABSTRACT:

Objective

To explore pre-analysis factors affecting the results of neuron-specific enolase (NSE) testing of serum samples, select optimal testing conditions, and reduce the false-positive rate of NSE.

Methods

Serum NSE levels were measured using the Roche e801 electrochemiluminescence immunoassay in healthy participants who underwent physical examinations at the Fourth Affiliated Hospital of Soochow University from September 2022 to December 2024, including both outpatients and hospitalized patients. Further, we analyzed the effects of various pre-analytical factors on the NSE positivity rate (NSE>16.3 ng/mL was judged to be NSE-positive) and the proportion of hemolysis index (H) ≥13 (H = 13 was equivalent to hemoglobin concentration of 13 mg/dl).

Results

This study f

SUBMITTER: Honghui T 

PROVIDER: S-EPMC12274762 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature