Induction of host genes by nested genes during <i>C. elegans</i> development.

ABSTRACT: Embryonic development relies on tightly controlled gene expression. In this study, we analyzed the transcriptional effect during C. elegans development of a striking genomic topology, the opposite nested configuration, where a gene is located in an intron of a host gene in the opposite direction. Using CRISPR genome engineering and single molecule FISH, we characterized the regulatory interactions between ceh-10, a transcription factor involved in neuronal specification, and its host polq-1, a DNA repair enzyme, showing that the nested gene induces transcription of a short version of its host in neurons. Extending our analysis to the hundreds of protein coding genes in opposite nested configuration and using single-cell RNA-seq data covering C. elegans embryogen

SUBMITTER: Soulavie F 

PROVIDER: S-EPMC12274878 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature