Protocol for isolating cervico-vaginal fluid cells from Macaca mulatta to study immunological and functional changes during pregnancy.

ABSTRACT: Cervico-vaginal fluid represents an important biological fluid due to its proximity to gestational tissues, such as the cervix, fetal membranes, and myometrium. Here, we present a protocol to isolate cervico-vaginal fluid cells from Macaca mulatta. We describe steps for cervico-vaginal fluid collection, cervico-vaginal cell isolation for nuclear staining and immunophenotyping by flow cytometry, and protein-level detection and quantification by ELISA. This protocol can provide insights into altered immunological changes at the cervico-vaginal interface.

SUBMITTER: Short D 

PROVIDER: S-EPMC12274921 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature