Chromatin landscape alteration uncovers multiple transcriptional circuits during memory CD8+ T-cell differentiation.

ABSTRACT: Extensive epigenetic reprogramming involves in memory CD8+ T-cell differentiation. The elaborate epigenetic rewiring underlying the heterogeneous functional states of CD8+ T cells remains hidden. Here, we profile single-cell chromatin accessibility and map enhancer-promoter interactomes to characterize the differentiation trajectory of memory CD8+ T cells. We reveal that under distinct epigenetic regulations, the early activated CD8+ T cells divergently originated for short-lived effector and memory precursor effector cells. We also uncover a defined epigenetic rewiring leading to the conversion from effector memory to central memory cells during memory formation. Additionally, we illustrate chromatin regulatory mechanisms underlying long-lasting versus transient transcription regulation d

SUBMITTER: Liu Q 

PROVIDER: S-EPMC12275094 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature