Extract of camellia seed cake ameliorates glycolipid metabolism disorder in mice through inhibiting ACOX1 activity.

ABSTRACT: Acyl-CoA oxidase 1 (ACOX1) plays a key role in glycolipid metabolism disorders. Camellia seed cake, a byproduct of oil production, was identified as a source of ACOX1 inhibitors. Under optimized conditions (58 % ethanol, 70 °C, 62 min), the extract exhibited an 83.55 % inhibition rate and an IC₅₀ of 18.88 mg/mL after purification. Among 138 compounds identified in the extract, 57 were flavonoids, with luteolin-4'-O-glucoside showing the highest binding affinity to ACOX1. In diabetic mice, the extract significantly reduced hepatic ACOX1 activity by 46.49 % and blood glucose levels by 25.76 %, and simultaneously decreased blood lipids and alleviated hepatic lipid accumulation. Oxidative stress was mitigated through reduced H₂O₂ production and enhanced antioxidant enzyme act

SUBMITTER: Chen B 

PROVIDER: S-EPMC12275135 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature