Efficient and simple protocol for mechanical isolation of foreskin-derived primary human dermal fibroblasts for replicative senescence studies.

ABSTRACT:

Background

An efficient and reproducible protocol was developed for the mechanical isolation of human dermal fibroblasts (HDFs) from the foreskin, providing a practical alternative to enzymatic methods. This protocol could be easily performed with limited resources by reducing the need for expensive reagents and complex procedures. Viable cells were successfully subcultured repeatedly for cellular senescence studies, with reproducibility ensured through a detailed description.

Results

This mechanical isolation method successfully generated HDFs with elongated spindle-shaped morphological characteristics that expressed the ACTIN protein, VIMENTIN protein, and SERPINH1 gene. HDFs survived through passage 8 (P8) during repeated subculturing.

Conclusions

The mechanical is

SUBMITTER: Khairani AF 

PROVIDER: S-EPMC12275237 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature