Taqman-probe-based multiplex real-time RT-QPCR for simultaneous detection of duck tembusu virus, novel duck reovirus, duck hepatitis a type 1 virus and duck hepatitis a type 3 virus.

ABSTRACT: Novel duck reovirus (NDRV), duck hepatitis A type 1 virus (DHAV-1), duck Tembusu virus (DTMUV), and duck hepatitis A type 3 virus (DHAV-3) caused substantial economic losses within the duck industry. In our research, we aimed to develop a TapMan probe-based multiplex quantitative reverse transcription polymerase chain reaction (RT-qPCR) method to enable the early detection of these four viruses in suspicious duck populations. The detection limits for DTMUV, NDRV, DHAV-1, and DHAV-3 were 8.08 × 10¹, 1.24 × 10², 6.03 × 10¹, and 1.88 × 10² copies/μL, respectively. Additionally, the DTMUV, NDRV, DHAV-1, and DHAV-3 were successfully detected. No positive signals were detected for duck enteritis virus (DEV), muscovy duck parvovirus (MDPV), goose parvovirus (GPV), avian influenza virus (AIV), and

SUBMITTER: Qiu Q 

PROVIDER: S-EPMC12275695 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature