UBE2J2 is essential for the progression of meiosis prophase I during spermatogenesis in mice.

ABSTRACT: The coordination of numerous proteins is necessary for spermatogenesis, including degradation through the ubiquitin-proteasome pathway. Ubiquitin binding enzyme E2 (UBE2J2) is involved in the degradation of endoplasmic reticulum-associated proteins, while its role in spermatogenesis remains unclear. We found that Ube2j2 ^-/- mice exhibit azoospermia and spermatocytes undergo meiotic arrest at the mid-pachytene stage. Examining homologous recombination (HR) markers indicated that HR intermediate complexes are unstable and fail to form crossovers in Ube2j2 ^-/- spermatocytes. Proteomics analysis uncovered an extensive suite of meiosis- and chromosome segregation-associated proteins unexpressed in mouse spermatocytes lacking functional UBE2J2. Our findings su

SUBMITTER: Yu X 

PROVIDER: S-EPMC12275893 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature