Identification of Small Open Reading Frame-Encoded Peptides in Glioma by an Optimized Proteomics Strategy.

ABSTRACT: Small open reading frame-encoded peptides (SEPs), translated from previously unannotated genomic regions, have emerged as important regulators in diverse physiological and pathological processes. While ribosome profiling and bioinformatics analysis can predict putative SEPs, mass spectrometry (MS) is the only method for their definitive identification. However, MS-based SEP detection faces significant challenges due to SEP's short length and low abundance. To address these limitations, we developed an ammonium formate-mediated C8 solid-phase enrichment (AmF-C8-SPE) strategy that significantly outperforms classic C8-SPE, yielding superior SEP identification with enhanced unique peptide ratios and sequence coverage. By coupling AmF-C8-SPE with fractionation and LC-MS/MS analysis of glioma sa

SUBMITTER: Zhang T 

PROVIDER: S-EPMC12275937 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature