A Novel Multivariate Analysis: Overturning Long-Held Beliefs About Non-Photochemical Quenching.

ABSTRACT: When light absorption exceeds photochemical quenching, plants activate non-photochemical quenching (NPQ) to dissipate excess energy as heat. Recently, we have developed a novel multivariate pipeline for NPQ induction analysis. Applying this pipeline to NPQ induction curves of several Arabidopsis thaliana NPQ genotypes, overturns the long-held belief that zeaxanthin (Zx) accelerates NPQ induction upon light-adaptation. We demonstrate that the observed acceleration is solely due to the action of PsbS. Our approach allows the synergistic inter-relationships between PsbS and Zx to be unambiguously explored. Specifically, we applied our analysis to dark- and light-adapted wild-type (wt), Zx-lacking (npq1), Zx-rich (npq2) and PsbS-lacking (npq4) A. thaliana. Only the PsbS-dependent quenching in

SUBMITTER: Ramakers LAI 

PROVIDER: S-EPMC12275998 | biostudies-literature | 2025 Jul-Aug

REPOSITORIES: biostudies-literature