SWI5-SFR1 reduces RAD51 recombinase extending units during filament assembly.

ABSTRACT: Homologous recombination is a key pathway for repairing DNA double-strand breaks with high fidelity. The assembly of recombinases on DNA to form nucleoprotein filaments is a crucial and tightly regulated step. This process requires the formation of a stable nucleus, followed by recombinase extension. The dynamic assembly and disassembly of recombinases directly affect recombination progression. Accessory proteins, such as SWI5-SFR1, regulate nucleation and extension steps, modulating filament stability and recombination efficiency. In this study, we extended our investigation to the rapid extension phase of RAD51 filament assembly. We found that mouse SWI5-SFR1 effectively reduces the dissociation probability of mouse RAD51 during filament extension, promoting more uniform filament growth.

SUBMITTER: Hu Y 

PROVIDER: S-EPMC12276003 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature