Untargeted CUT&Tag reads are enriched at accessible chromatin and restrict identification of potential G4-forming sequences in G4-targeted CUT&Tag experiments.

ABSTRACT: G-quadruplex DNA structures (G4s) form within single-stranded DNA in nucleosome-free chromatin. G4s modulate gene expression and genomic stability, so high-throughput, genome-wide mapping of G4s has generated strong research interest and methodological innovation. Recently, the Cleavage Under Targets and Tagmentation (CUT&Tag) method has been adapted to map G4s using an antibody, a nanobody, and G4-binding small molecules to target Tn5 tagmentation to G4s. These novel methods have generated high-resolution maps of G4s, but we have observed a strong colocalization between untargeted and G4-targeted CUT&Tag signal enrichment, leading us to wonder whether this colocalized signal enrichment would impact G4 mapping using these methods. We observed that the genome-wide signal distribution of unt

SUBMITTER: Thompson MD 

PROVIDER: S-EPMC12276005 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature