Assessment of current use pesticides in flowers, pollen provision, and wild bees: HPLC-ESI-MS/MS method development and field implementation.

ABSTRACT: Synthetic pesticide use is a major driver of pollinator declines in agricultural landscapes. To understand the impact of pesticides, it is essential to quantify residues in food resources and in insects themselves. We developed simple, fast, and cost-effective multiresidue methods for the simultaneous quantification of up to 83 current use pesticides (CUPs) in flowers (0.5 g sample weight) and 71 CUPs in pollen provision (0.1 g sample weight) via liquid chromatography-tandem mass spectrometry. Additionally, methods were developed for individual wild bees (Osmia bicornis), enabling the analysis of 65 CUPs in 0.02 g samples (females) and 45 CUPs in 0.01 g samples (males). The extractions used acidified acetonitrile (2.5% formic acid), with phase separation assisted by ammonium formate and cl

SUBMITTER: Honert C 

PROVIDER: S-EPMC12276102 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature