Green sample preparation for anthocyanin extraction from purple corn: analytical evaluation of pressurized liquid and ultrasound-assisted extraction using sustainable solvents.

ABSTRACT: This study presents an analytical comparison of two extraction techniques-pressurized liquid extraction (PLE) and ultrasound-assisted extraction (UAE)-for the recovery of anthocyanins from purple corn using sustainable solvents (o-phosphoric acid, ethanol, and water). Twelve anthocyanins were identified using high-performance liquid chromatography with ultraviolet detection and tandem mass spectrometry, confirming method specificity and selectivity. Both extraction methods were optimized using multivariate experimental designs and validated following U.S. Food and Drug Administration guidelines for analytical methods in food matrices. The protocols demonstrated excellent linearity (coefficient of determination ≥ 0.9992), low detection limits (0.30-1.70 mg/kg), and high precision and accura

SUBMITTER: Custodio-Mendoza JA 

PROVIDER: S-EPMC12276115 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature