In vitro evaluation of tolerance ability of cross-kingdom biofilm towards oral dynamic fluctuations.

ABSTRACT:

Objectives

The aim of this study was to investigate the resilience of Streptococcus mutans (S. mutans) and Candida albicans (C. albicans) cross-kingdom biofilms in response to environmental stresses.

Materials and methods

The growth kinetics of S. mutans, C. albicans, and their co-culture in planktonic form were assessed using a BioScreen system. Biofilms were established on 96-well plates for a duration of 48 h, after which microbial counts were determined. The biofilms were then subjected to different stress conditions, including oxidative, acid, osmotic, and heat stress, for 2 h. The survival and structural integrity of the biofilms were evaluated through colony-forming unit (CFU) counting and fluorescence microscopy, respectively. Additionally, the transcriptional levels

SUBMITTER: Li Y 

PROVIDER: S-EPMC12276691 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature