Magnetic bead-based electrochemical surrogate virus neutralization test for quantification of antibody neutralizing efficiency.

ABSTRACT: Rapid and inexpensive quantification of antibody neutralizing efficiency is currently limited due to the use of live virus and strict biosafety level requirements necessary to perform a plaque reduction neutralization test (PRNT), severely restricting efficient evaluation of vaccine efficacy, establishment of correlates of protection, and determination of individual immune status for many viral diseases. Based on the surrogate virus neutralization test (sVNT), we demonstrate a magnetic bead-based electrochemical sVNT (esVNT) for sensitive quantification of SARS-CoV-2 antibody neutralization efficiency in under 15 min using a portable potentiostat. By utilizing magnetic beads as a solid phase for the capture probe and chronoamperometric detection, we show increased capture efficiency of rea

SUBMITTER: Bellows RA 

PROVIDER: S-EPMC12276914 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature