Genome-wide association study identifies candidate SNP markers and genes associated with low nitrogen tolerance in <i>Brassica napus</i>.

ABSTRACT: Low nitrogen (LN) stress is a major limiting factor affecting crop growth and productivity. Understanding the genetic basis of LN tolerance is essential for improving nitrogen use efficiency in Brassica napus. A genome-wide association study (GWAS) was conducted on a panel of 275 B. napus accessions using a semi-automated hydroponic system to evaluate five seedling traits-leaf number (NL), shoot length (SL), root length (RL), shoot fresh weight (SFW), and root fresh weight (RFW)-under LN conditions. The system ensured environmental uniformity and high-throughput phenotyping. Significant phenotypic variation was observed across accessions, and correlation analysis suggested that RFW and SFW are key traits associated with LN tolerance. GWAS identified 71 significant SNPs, with

SUBMITTER: Chen J 

PROVIDER: S-EPMC12277277 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature