Plasma Proteomic Profiling of a Group of Anxious Dogs by LC-MS/MS: A Case-Control Study.

ABSTRACT:

Purpose

Anxiety is the most common underlying cause of behavioral problems in dogs, which remain a top reason for relinquishment and euthanasia. Despite its high prevalence, anxiety is often underdiagnosed, partly due to a limited understanding of biological processes and absence of diagnostic biomarkers. Our study aims to address this knowledge gap.

Experimental design

Plasma from 10 anxious and 10 matched control dogs were analyzed following a label-free quantitation proteomics workflow based on data-dependent acquisition using a Thermo Q Exactive Plus coupled to an EASY-nLC 1200, Vanquish UHPLC, or Evosep One. Data were processed with Proteome Discoverer 2.4 (Thermo), Perseus (Max Planck Institute), Cytoscape and other bioinformatic tools.

Results

Between 279 and 3

SUBMITTER: Gaither C 

PROVIDER: S-EPMC12278046 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature