Integrated proteomic and targeted Next Generation Sequencing reveal relevant heterogeneity in lower-grade meningioma and ANXA3 as a new target in NF2 mutated meningiomas.

ABSTRACT:

Background

Meningiomas, the most common primary brain tumours, are classified by the World Health Organization (WHO) into grades 1, 2, and 3. Some grade 1 tumours exhibit increased clinical aggressiveness, with the biallelic mutation of NF2 being the most frequently reported.

Methods

In our study, we analysed the most common driver mutations (NF2, AKT1, KLF4, and TRAF7) in meningioma by genomics describing co-occurrences and new mutations. Furthermore, tumour tissue bearing the driver mutations was analysed by proteomics. The relevance of the specific target found in the most common driver mutation in meningiomas (NF2) was validated in vitro using both lower and higher-grade meningioma and further, the higher-grade meningioma was analysed in vivo using an NOD scid gamma (NSG

SUBMITTER: Shah M 

PROVIDER: S-EPMC12278414 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature