Osteocalcin deficiency attenuates neointima formation after vascular injury in rat.

ABSTRACT:

Background

The bone-specific protein osteocalcin (OCN) is an established marker of vascular calcification and vascular smooth muscle cell (VSMC) osteogenic trans-differentiation. Recent studies have revealed the regulatory function of externally administered OCN on atherosclerosis and vascular remodeling. However, the role of OCN in VSMCs involved in cell migration and neointima formation has not been clearly described.

Methods

Rat carotid-artery balloon-injury was performed to induce neointima formation. OCN expression was detected in injured carotid arteries by western blot and immunofluorescence staining. Rat primary cultured VSMCs were treated with tumor necrosis factor-α (TNF-α) and platelet-derived growth factor-BB (PDGF-BB). OCN overexpression was induced by virus inf

SUBMITTER: Yi M 

PROVIDER: S-EPMC12278516 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature