Clonevdjseq: A workflow and bioinformatics management system for sequencing, archiving, and analysis of VDJ sequences from clonal libraries.

ABSTRACT:

Background

Advances in next-generation sequencing technologies have facilitated extensive analysis of B cell and T cell receptor (BCR/TCR, respectively) sequences from monoclonal hybridoma libraries, single B cells, and single T cells, generating vast amounts of important data pertaining to antigen recognition. However, existing workflows and bioinformatics tools often lack the flexibility and scalability needed to handle large clonal level datasets effectively. An initial system and hybridoma dependent version of this code was distributed as part of the NeuroMabSeq publication, but clonevdjseq aims to be a technical addendum for broader system compatibility and enhanced modeling.

Results

We present clonevdjseq, an integrated and accessible software solution leveraging nextf

SUBMITTER: Mitchell K 

PROVIDER: S-EPMC12278597 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature