Exploring the influence of sex and age on the quality and flavor of Huai goat longissimus thoracis et lumborum through transcriptomic and metabolomic analyses.

ABSTRACT:

Background

The aim of this study was to investigate the effects of age and sex on the composition of fatty acids, amino acids, and vitamins in the longissimus thoracis et lumborum (LTL) of Huai goats.

Methods

The longissimus thoracis et lumborum tissues of eight Huai goats were collected postslaughter and analyzed for amino acids, fatty acids, and vitamins by gas chromatography‒mass spectrometry (GC‒MS) and standard methods. RNA sequencing (RNA-Seq) was conducted to identify differentially expressed genes (DEGs) across different ages and sexes. Metabolite profiling was performed by liquid chromatography‒mass spectrometry (LC‒MS) to detect and quantify metabolites.

Results

This study revealed significant differences in amino acid and fatty acid profiles between male an

SUBMITTER: Han H 

PROVIDER: S-EPMC12278664 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature