Biogenic silver nanoparticles synthesized from Pseudomonas fluorescens-mediated olive cake waste: antimicrobial, larvicidal activity against Culex pipiens and cytotoxicity assessment.

ABSTRACT: This study presents an eco-friendly approach for synthesizing silver nanoparticles (AgNPs) using olive cake hydrolysate (OCH), produced through microbial fermentation of olive cake waste by Pseudomonas fluorescens. The OCH was analyzed by gas chromatography-mass spectrometry (GC-MS), revealing the biotransformation of olive cake components into bioactive compounds, including 24-norursa-3,12-diene, methyl esters of 9,12-octadecadienoic acid and 9-octadecenoic acid, and α-sitosterol. The biosynthesized olive cake hydrolysate-silver nanoparticles (OCH-AgNPs) were characterized using ultraviolet-visible (UV-Vis) spectroscopy to confirm surface plasmon resonance at 420 nm; Fourier-transform infrared (FTIR) spectroscopy to identify the involvement of hydroxyl and carbonyl functional groups; X-ra

SUBMITTER: Abu-Hussien SH 

PROVIDER: S-EPMC12278677 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature