Fine resolution of the N-terminal IgE-binding epitope of Ara h 2: Discovery of variants with enhanced IgE binding.

ABSTRACT:

Background

IgE binding to linear peptides from the N-terminal region of Ara h 2 (epitope 1) is associated with the achievement of sustained unresponsiveness in young children receiving oral immunotherapy and may be important for cross-reactivity between peanuts and tree nuts. This region is also part of the binding site for neutralizing IgG monoclonal antibodies associated with sustained unresponsiveness following oral immunotherapy.

Objective

We sought to perform alanine scanning of this epitope to determine the importance of individual amino acids and then amino acid scanning to screen for sequences with enhanced binding of IgE.

Methods

A streptavidin IgE ELISA with biotinylated peptides was used to measure the binding of IgE to full-length and truncated peptides to

SUBMITTER: Bernstein JS 

PROVIDER: S-EPMC12278769 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature