Blood group O expression in normal tissues and tumors.

ABSTRACT: The H antigen (O blood group), the precursor to A and B blood groups, is expressed on human erythrocytes and other cells (e.g., endothelial cells). Remarkably, the specific expression of the H antigens is uncertain due to the lack of specific reagents for its detection. Here, we describe two monoclonal antibodies (mAbs), Tn4-31L and OmcFL3-02, generated by immunizing sea lampreys with human cells. Both mAbs are highly specific in comparison with the lectin Ulex europaeus agglutinin-I (UEA-I). We identified expression of H antigens in mammalian glycoproteins, human cells, and normal and malignant human tissues, discovering that different types of human carcinomas exhibit unexpected H antigen expression. Interestingly, H antigen detection in many cases was distinctly elevated by enzymatic de

SUBMITTER: Tulin EKC 

PROVIDER: S-EPMC12278781 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature