Incorporation of iPSCs together with TERT-immortalized keratinocytes and fibroblasts into reconstructed human gingiva enhances phenotype of gingival epithelium.

ABSTRACT: The oral mucosa plays an important role in maintaining oral and systemic health by protecting the body from harmful environmental stimuli and pathogens. Current reconstructed human gingiva models (RhG) serve as valuable testing platforms for safety and efficacy testing of dental materials, however they lack important phenotypic characteristics typical of the gingival epithelium. We aimed to determine whether incorporating induced pluripotent stem cells (iPSCs) into the hydrogel of a cell-line RhG (reconstructed epithelium on fibroblast-populated-hydrogel) would improve its phenotype. Immortalized human gingival fibroblasts were resuspended with and without iPSCs in collagen-fibrin hydrogels and gingival keratinocytes were seeded on top of the hydrogels to construct RhGs. RhGs were cultured

SUBMITTER: Brueske LL 

PROVIDER: S-EPMC12279103 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature