Heterologous prime-boost immunization based on a human adenovirus 5 vectored containing Trichinella spiralis Cystatin-like protein elicits protective mucosal immunity in mice.

ABSTRACT: Trichinellosis is a globally distributed zoonotic parasitic disease. The Trichinella infective larvae migrate through the intestine after ingestion and settle in muscles, thus intestinal mucosal immunity plays a vital role against early infection with Trichinella. In this study, a recombinant adenovirus vector expressing the cysteine protease inhibitor of Trichinella spiralis (rAd5TsCLP) was constructed and combined with the recombinant protein rTsCLP in a heterologous prime-boost regimen. The regimen elicits strong, specific, and neutralizing antibodies in BALB/c mice, significantly enhancing cellular immunity through Th1 (IFN-γ, TNF-α) and Th2 (IL-13, IL-4) cytokine production in the peripheral blood, spleen, and cervical lymph nodes, driven by the activation of CD4+ and CD8+ T-cells. No

SUBMITTER: Xu N 

PROVIDER: S-EPMC12279143 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature