Exploring the impact of forage-to-concentrate ratios on the ruminal bacteriome in vitro focusing on ciliate-associated bacteria.

ABSTRACT: Ruminal ciliates are linked to methane production and nitrogen utilization efficiency in ruminants due to their association with other ruminal microorganisms. However, research on the specific interplay between ruminal bacteria and ciliates is still limited, particularly in different dietary conditions. This study examines the effect of the forage-to-concentrate (F:C) ratio on the ruminal bacteriome in vitro, focusing on bacteria associated with Isotricha spp. and small entodinia. The rumen fluid used as the inoculum for this experiment was collected from two cannulated Hanwoo cows. Dietary treatments included high-forage (HF, F:C of 7:3), high-concentrate (HC, F:C of 3:7), and control (CON, F:C of 5:5). After 24-hour incubation, fractions for entodinia-associated bacteria (EAB), Isotricha

SUBMITTER: Kim G 

PROVIDER: S-EPMC12279964 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature