Developmental specificity of recruitment of TBP to the TATA box of the human gamma-globin gene.

ABSTRACT: It is unclear whether the core promoter is involved in developmental regulation. To address this question, we mutated the TATA box of the human gamma-globin gene, produced transgenic mice, and examined the effect of the mutation during the course of mouse development. In our test system, the gamma-globin gene is expressed at similar levels in the embryonic and adult erythroid cells. The TATA box mutation dramatically reduced expression of the gamma-globin gene in the adult but not in embryonic erythroid cells. In addition, the disruption of the gamma TATA box significantly reduced the recruitment of TATA box-binding protein (TBP) in the adult cells, but not in embryonic cells, suggesting that the recruitment of TBP to the gamma gene promoter is developmentally specific. Similarly, the recr

SUBMITTER: Duan ZJ 

PROVIDER: S-EPMC122800 | biostudies-literature | 2002 Apr

REPOSITORIES: biostudies-literature