HIF-1α alleviates ferroptosis in ulcerative colitis by regulation of GPX4.

ABSTRACT: Ferroptosis is an iron-dependent form of regulated cell death induced by the lethal accumulation of lipid peroxidation, while the precise mechanism of ferroptosis in the pathogenesis of ulcerative colitis (UC) remains to be elucidated. This study aimed to explore the potential effect of hypoxia inducible factor-1α (HIF-1α) on ferroptosis in intestinal epithelial cells (IECs) in UC. The relationship between ferroptosis and HIF-1α was initially investigated using clinical UC colon samples. In vitro and in vivo models of acute intestinal inflammatory response were constructed using lipopolysaccharide (LPS) and dextran sulfate sodium (DSS), respectively. The effect of HIF-1α on ferroptosis in UC was determined by establishing HIF-1α overexpression (HIF1A-OE) or knockdown (shHIF1A) IEC lines, a

SUBMITTER: Hu W 

PROVIDER: S-EPMC12280037 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature