On the Difficulty to Detect Carbapenem Resistance in the Environment: Characterisation of Escherichia coli With Reduced Carbapenem Susceptibility Isolated in a French River.

ABSTRACT: During an 18-month longitudinal study, bi-monthly water samples were taken upstream and downstream of a watershed. In order to detect carbapenem-resistant E. coli, the CHROMIDCarba medium was used. Of the 863 isolates collected from 144 samples, E. coli identification was confirmed for only seven of them, isolated on the same day. For six isolates, a slightly reduced susceptibility to carbapenems was observed. The results of the whole genome sequencing indicate that the six isolates belong to the same clone (O8:H7, ST196). Furthermore, a mutation of the ompC porin coupled with the presence of the bla_CMY-2 gene, on an IncI1 plasmid, would be at the origin of the reduced sensitivity of these strains to carbapenems. This type of mechanism has already been described in human clinica

SUBMITTER: Laetitia LD 

PROVIDER: S-EPMC12280049 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature