Nanopore full length 16S rRNA gene sequencing increases species resolution in bacterial biomarker discovery.

ABSTRACT: Discovery of disease-related bacterial biomarkers could be a useful approach for early prevention or diagnosis of various afflictions, such as colorectal cancer. This typically involves analyzing small regions of the 16S rRNA gene (e.g. V3V4) through short-read technologies like Illumina, obtaining genus-level results. However, recent developments in third-generation sequencing, such as Oxford Nanopore Technologies (ONT)'s new R10.4.1 chemistry and its improved basecalling models, are beginning to allow for a more complete and accessible species-level analysis through full-length 16S rRNA gene sequencing (spanning regions V1-V9). Thus, the goal of this study was to compare and evaluate both approaches, using colorectal cancer biomarker discovery as a representative case. This was achieved

SUBMITTER: Aja-Macaya P 

PROVIDER: S-EPMC12280133 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature