Hierarchical metal exposure in umbilical cord blood in relation to oxidative stress and AMPK/NF-κB signaling in preterm membrane rupture.

ABSTRACT: To investigate the correlation between metal element levels, AMPK(AMP-activated protein kinase) and NF-κBp65(Nuclear factor kappa-B p65) and PPROM(preterm premature rupture of membranes). 20cases of PPROM,33cases of TPROM(Term premature rupture of membranes), and 33cases of nPROM were selected, totaling 86patients.Metal element content in umbilical cord blood serum was measured using ICP-MS(Inductively Coupled Plasma Mass Spectrometry).HE(Hematoxylin-eosin) staining was utilized to observe histological changes in the placentas of the three groups.IHC(Immunohistochemistry) and Western Blot were applied to detect the expression levels of AMPKα and NF-κBp65 in the placental tissues. The levels of Mg, Fe, and Mo in the PPROM group were significantly lower than those in the nPROM group and TPRO

SUBMITTER: Lu S 

PROVIDER: S-EPMC12280158 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature