Comparative LC-MS Proteomics of Quinoa Grains: Evaluation of Bioactivity and Health Benefits by Combining In Silico Techniques With In Vitro Assays on Colorectal Adenocarcinoma Cells.

ABSTRACT: In the present study, we investigated the potential biological effects of proteins and peptides extracted from four different commercial quinoa grain varieties: Black (B), Red (R), White (W), and Royal White (RO), using Caco-2 cells as a proliferation model. Concentration-response curves were constructed to evaluate cytotoxicity and antiproliferative effects. Interestingly, peptides exhibited higher cytotoxicity than proteins, particularly in R and RO quinoa varieties. Based on these findings, we used a data mining approach to identify and compare the cytotoxic proteins in the four quinoa varieties. Using their relative abundance, we successfully classified R and RO quinoa as a cohesive group using classification models. To gain deeper insights into the biological effects on cells, we comp

SUBMITTER: Zaccarelli A 

PROVIDER: S-EPMC12280847 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature